Glutamate and glutamine in cerebrospinal fluid and serum from healthy volunteers - analytical aspects
Summary
Type: HPLC Application #: 107680
Catalog#: 720120.46 - MN HPLC Column, Nucleosil C18, 100A, 5um, 4.6x150mm
Detection: fluorescence, excitation 330nm, emission 455nm Conditions: eluent: A) phosphate buffer pH 6.0, B) methanol, gradient from 20 to 70% B) in 20min flow rate 1.2ml/min Authors: Alfredsson G Source: J Chromatogr 424 (1988) 378 - 384
Details
Legend: Fig. 1. HPLC of amino acids in CSF as OPT derivatives, 1. cysteic acid (int.
std.), 2. unknown, 3. glutamate, 4. asparagine, 5. serine, 6. glutamine, 10µl
injected.
also see G. Alfredsson and G.